Microbial Engineering Research Associate

Synvivia seeks a full-time Microbial Engineering Research Associate to help build engineered organisms that can be controlled with synthetic small molecules. This role requires a focused individual with a detail-oriented mentality and a strong work ethic.

This position is focused on DNA assembly, molecular cloning, genome engineering, robotic high-throughput screening, and phenotypic analysis. Primary responsibilities include fabrication of DNA constructs using a combination of PCR, restriction enzyme digestion, ligation, transformation, DNA extraction, and DNA sequencing. Secondary responsibilities include general laboratory management.

Required Skills:

Molecular cloning and DNA assembly with E. coli PCR DNA electrophoresis DNA purification and cleanup (i.e. PCR cleanups, gel purification) Restriction digestion, ligation (or other assembly techniques) DNA transformation Plating microbial transformation on appropriate conditions Plasmid DNA purification (i.e. mini prepping) DNA sequence sample preparation (sanger) and analysis Excellent sterile technique Excellent pipette and liquid-handling technique Excellent record-keeping

Preferred Skills:

SOEING, EIPCR, error prone PCRs, and primer design Golden gate assembly Genome integration